Endocrine Abstracts

Though LC-MS/MS is replacing immunoassays for major glucocorticoids and androgens routine assessment, the measurement of other sex steroids and precursors is still challenging and available only in experienced research laboratories. Our aim was to develop a simple-prep and rapid 2D-LC-MS/MS method for the simultaneous measurement of estrone (E1), estradiol (E2), dihydrotestosterone (DHT) and 17OHpregnenolone.Serum volumes of 300ul were spiked with intern...

Steroid profiling by liquid chromatography–tandem mass spectrometry (LC–MS/MS) offers an accurate and simultaneous detection of a panel of steroids with several advantages over traditional assays. However, only a few studies have investigated the steroid profiling in sporadic adrenocortical adenomas. Our aim was therefore to investigate steroid secretion by LC–MS/MS in patients with non-secreting adrenocortical adenomas (NSA) and subclinical hypercortisolism (SH...

Hyperandrogenic disorders cause psychological implication in young girls, limiting the quality of life. Salivary cortisol (SalF) testing was proved to be useful in the evaluations of acute stress responses. Aim of this study was to investigate SalF responses to a stressor event in late adolescent females. We selected 165 drug-free females aged 16–19 years from a cross-sectional epidemiological study. Saliva was collected in the morning before and after a stressor event co...

Introduction: The relationship between food intake and hypothalamic–pituitary–adrenal (HPA) axis has been studied in the development of visceral adiposity and obesity. The HPA axis may interact with the reward system, thus influencing food choices. On the other hand, it has been shown that macronutrients differently modulate the HPA axis activity.The aim of this study was to evaluate the dietary habits in subjects with adrenal masses (adenoma o...

Background: PD-L1 represents a novel class of drugs for treatment of a broad spectrum of malignancies including onco-hematologic diseases. This treatment is associated with several immuno-related adverse events (irAEs). Thyroid diseases and diabetes are among the most common PD-L1 endocrine-irAEs reported in solid cancers.Aims: To describe the frequency, the onset and the characteristics of PD-L1 endocrine-irAEs in patients with hematologic malignancies....

Turner syndrome (TS), one of the most frequent chromosomal aberrations in females, is characterized not only by structural abnormalities (somatic and congenital) but also by acquired comorbidities, such as metabolic disorders, cardiovascular alterations, autoimmune diseases, osteoporosis, and malignancies. The prevalence of structural abnormalities that usually complicate TS at diagnosis is well known. However, the prevalence of acquired complications, that may appear at any a...

Introduction: Women with polycystic ovary syndrome (PCOS) are considered to have increased risk of cardiovascular disease (CVD), even though the syndrome encompasses hugely varying phenotypes. Research into CVD prevention demonstrated a variety of biochemical and ultrasound (US) markers predicting risk in the general population, but those are sparsely utilised for PCOS, in part due to the patients’ therapeutic goals.Aim: To evaluate if PCOS type or...

Background: In the last years, Pembrolizumab has been used to treat solid and lymphatic neoplasms, showing improved patients’ survival. Due to its mechanism of action, Pembrolizumab may be responsible of several immune-related adverse events (irAEs). The most frequent endocrine-irAEs reported are thyroid diseases.Aims: The aim of this study was to evaluate differences in thyroid disease incidence and presentation between patients treated for lung c...

The use of LC–MS/MS quantitative methods in clinical and research laboratories is becoming a well established reality that may overcome specificity and accuracy limits of immunoassays. Nevertheless, among different LC–MS/MS methods an agreement on the values that are to be considered normal or pathologic has not been reached yet, and this is mainly due to differences in LC–MS/MS methods, to the poor or absent availability of reference material and to the selecti...

The endocannabinoid system is involved in modulating many metabolic and behavioural processes. Due to the instable nature of endocannabinoids in biological fluids and to the poor sensitivity of traditional analytical technologies, little is known about physiological circulating levels of these lipid mediators. We developed and validated an ID-LC-MS/MS method for the simultaneous measurement of four endocannabinoids: anandamide (AEA), 2-arachidonoylglycerol (2-AG), palmitoyl-et...